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MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates
GSA_VA

Catalog No. wbluf0500 Shop All MilliporeSigma Products
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Quantity:
500 mL
Product Type:
HRP Substrate
Sensitivity:
∼1 to 3 pg
∼400 fg
∼6 pg
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Catalog No. Quantity Product Type Sensitivity
WBLUF0500 500 mL HRP Substrate ∼400 fg
WBLUR0500 500 mL HRP Substrate ∼1 to 3 pg
WBLUC0500 500 mL HRP Substrate ∼6 pg
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Catalog No. WBLUF0500 Supplier MilliporeSigma™ Supplier No. WBLUF0500
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Description

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

  • Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

  • Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
  • Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
  • Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

  • Immobilon Classico premixed reagent for everyday detection needs
  • Immobilon Crescendo premixed reagent for detection in the picogram range
  • Immobilon Forte premixed reagent for detection in the femtogram range

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TRUSTED_SUSTAINABILITY

Specifications

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
Quantity 500 mL
Sensitivity ∼400 fg
Content And Storage 18°C to 24°C
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